Application

Research CategoryStem Cell Research

This Anti-DPPA-2 Antibody, clone 6C1.2 is validated for use in WB for the detection of DPPA-2.

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DPPA-2 (developmental pluripotency associated-2) is a novel Oct-4-related protein that is localized to the nucleus of mouse fetal germ cells. DPPA-2 has binding sites for both POU and Sox-2 protein domains and is expressed primarily in the developing germ line following gastrulation. These associations suggest that this protein participates in a very strong supportive role in maintaining cell pluripotentiality. In addition to its role as a pluripotency marker, more recent research using cancer cells suggests that DPPA-2 may also act as an embryo-cancer antigenR12;pointing to the presence of abnormal cell growth and the formation of cancerous cells.

Immunogen

Full length recombinant mouse DPPA-2 protein.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2a_κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) and
150 mM NaCl with 0.05% sodium azide.

Quality

(Based upon representative data from a previous lot).
Western Blotting: Recommended working concentration is 2 µg/mL.

Specificity

This monoclonal antibody recognizes the mouse DPPA-2 protein.

Reactivity with other species has not been tested.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Target description

~34 kDa